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KMID : 1144820180240030275
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대한의생명과학회지
2018 Volume.24 No. 3 p.275 ~ p.279
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Immunofluorescence Microscopic Evaluation of Tight Junctional Proteins during Enterotoxigenic Bacteroides fragilis (ETBF) Infection in Mice
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Hwang Soon-Jae
Kang Yeo-Wool
Jo Min-Jeong
Kim Sung-Hoon
Cho Won-Gil
Rhee Ki-Jong
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Abstract
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Inflammatory bowel disease (IBD) is increasing in prevalence in developed countries but the cause of this increase is unclear. In animal models of IBD and in human IBD patients, alterations in the tight junctional proteins have been observed, suggesting that the intestinal microflora may penetrate the underlying colonic tissue and promote inflammation. Enterotoxigenic Bacteroides fragilis (ETBF) causes inflammatory diarrhea in human and is implicated in inflammatory bowel diseases. However, it is unclear whether alterations in tight junctional proteins occur during ETBF infection in mice. In this brief communication, we report that ETBF infection induces up-regulation of claudin-2 and down-regulation of claudin-5 through B. fragilis toxin (BFT) activity in the large intestine of C57BL/6 mice. In contrast, BFT did not induce changes in tight junctional proteins in the HT29/C1 cell line, suggesting that analysis of biological activity of BFT in vivo is important for evaluating ETBF effects.
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KEYWORD
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Enterotoxigenic Bacteroides fragilis, Tight junction, Claudin, Bacteroides fragilis toxin
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